Catalog Number - 40031-10P

40031-10P Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering a unique mixed‑mode retention mechanism that combines reversed phase, ion exchange, and HILIC interactions in a single column.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid functional group, enabling:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This combination provides enhanced selectivity for basic, polar, and ionizable compounds, making it especially valuable for complex matrices where traditional C18 or ion exchange phases are limited.

The TYPE‑C silica hydride base delivers significant performance advantages:

• Direct silicon–carbon bonding for superior chemical stability

• Operates at very low pH (<2) without damage

• Faster equilibration compared to traditional silica columns

• Reduced sensitivity to mobile phase moisture in HILIC mode

100 mm Length – Balanced Resolution for Routine HPLC

The 100 mm length provides an optimal balance between separation efficiency and analysis time in conventional HPLC workflows.

• Greater resolution than 50 mm columns

• Faster run times than longer formats

• Improved separation of closely related compounds

✅ Recommended for routine HPLC methods when 50 mm columns lack sufficient resolution

Flow Rate & System Requirements (4.6 mm ID, 4 µm)

✅ Fully compatible with standard HPLC systems

• Typical flow rate range: ~1.0 – 1.5 mL/min

• Moderate backpressure

• Compatible with UV and MS detectors

• Suitable for conventional analytical systems

✅ Best Practice: Use sample filtration and a guard column to extend column life and maintain performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Ideal for Complex Matrices

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Retention can be tuned using pH and ionic strength

✅ Ideal when C18 or traditional ion exchange methods do not achieve desired separation

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under aggressive conditions including low pH

• Resistant to hydrolysis

Faster Equilibration

• Reduces cycle time between runs

No Strict Moisture Control (HILIC Mode)

• Simplifies method development

Long Column Lifetimes

• Durable chemistry for routine use

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Standard HPLC Compatibility (4 µm)

• No need for UHPLC system

• Lower pressure than sub‑2 µm columns

Balanced Performance

• Strong efficiency with manageable backpressure

Typical Applications

• Routine analytical HPLC methods

• Complex matrix separations

• Basic and positively charged compounds

• Polar and ionizable analytes

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Compatible with standard HPLC systems

• Suitable for hydrophilic and hydrophobic compounds

• pH control is critical for WCX selectivity